anti cdx2  (R&D Systems)

Journal: bioRxiv

Article Title: Sex chromosomes shape the transcriptional landscape of the preimplantation mouse embryo

doi: 10.1101/2024.11.26.625424

Figure Lengend Snippet: a . Left: total cell number in E3.5 embryos (X m Y n=57, X m X p n= 31, X p O=10, X p O Xist Δ n= 14 embryos); mean value indicated by a red diamond; p -value generated using Student’s t-test. Right: Z-projection of confocal sections (1µm thickness) of E3.5 embryos stained for CDX2 (outside cells), and NANOG (inside cells); scale bar: 20µm. b . UMAP showing E3.5 X p O (blue) and X p O Xist Δ (magenta) cells relative to all other genotypes at E3.5 (green) and other time points (grey). c . Proportion of cells within each cluster (uncommitted, ICM, TE) per genotype at E3.5, derived from UMAP in b . d . Normalised expression of early ( Cdx2 ) and late ( Eomes , Elf5 ) TE genes in E3.5 TE cluster cells. e . X-to-autosome (X:A) ratios of X p O and X p O Xist Δ embryos; P values derived from Student’s t-test; one point was excluded as it is outside the represented range. f . Left: quantitation of inactive X-associated ubiquitylated histone H2A at lysine-119 (uH2A) staining in E3.5 embryos (X m Y n=12, X m X p n=10, X p O n=4 embryos); mean value indicated by a red diamond. Right: Z-projection of confocal sections (1µm thickness – total thickness of 10 and 4.5µm respectively) of E3.5 embryos showing uH2A staining. Presence and absence of inactive X-associated uH2A shown by magenta and white circles, respectively. Inserts show a representative image of cells without and with a uH2A cloud. g . E10.5 embryo weights in mg (log 10 ) by genotypes; mean value indicated by a red diamond; P values derived from Wilcoxon test.

Article Snippet: Primary antibodies used in this study were: rabbit anti-H2AK119ub1 (Cell Signaling, 8240, 1:500), rabbit anti-Nanog (Cosmo Bio, RCAB001P, 1:500), goat anti-Cdx2 (R&D Systems, AF3665-SP, 1:500).

Techniques: Generated, Staining, Derivative Assay, Expressing, Quantitation Assay